RAD51 Antikörper (AA 1-338)

Produktnummer ABIN7468272

Dieses Maus Monoklonal-Antikörper erkennt spezifisch RAD51 in WB, IHC, IP, IHC (p), IF, ICC und PLA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für RAD51 Antikörper (AA 1-338) (ABIN7468272)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAD51 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Proximity Ligation Assay (PLA)

Güteklasse: KO Validated

Klon: 14B4

Produktdetails anti-RAD51 Antikörper

Bindungsspezifität: AA 1-338

Kreuzreaktivität: Huhn, Human, Maus, Ratte

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Full length (amino acids 1-338) Rad51 expressed in E. coli.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Neuro2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2 Validation: KO/KD, Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, No Preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Andere Bezeichnung: RAD51 recombinase

Hintergrund:

Synonyms: RAD51 recombinase , BRCC5 , FANCR , HRAD51 , HsRad51 , HsT16930 , MRMV2 , RAD51A , RECA

Background: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Molekulargewicht: 37 kDa

Gen-ID: 5888

UniProt: Q06609

Pathways: DNA Reparatur