FEN1 Antikörper (AA 1-380)

Produktnummer ABIN7464723

Dieses Maus Monoklonal-Antikörper erkennt spezifisch FEN1 in WB, IF, IP, ICC und ChIP. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für FEN1 Antikörper (AA 1-380) (ABIN7464723)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FEN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Güteklasse: KO Validated

Klon: 4-00E-07

Produktdetails anti-FEN1 Antikörper

Bindungsspezifität: AA 1-380

Kreuzreaktivität: Human, Maus

Aufreinigung: Protein G purified

Immunogen: Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: MCF7

Validation: KO/KD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.73 mg/mL

Buffer: PBS, No Preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Andere Bezeichnung: flap structure-specific endonuclease 1

Hintergrund: Flap structure-specific endonuclease 1 , FEN-1 , MF1 , RAD2,The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions. [provided by RefSeq, Jul 2008]

Molekulargewicht: 43 kDa

Gen-ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA