CHEK2 Antikörper

Produktnummer ABIN7464217

Der Maus Monoklonal Anti-CHEK2-Antikörper wurde für WB und IP validiert. Er ist geeignet, CHEK2 in Proben von Human zu detektieren.

Kurzübersicht für CHEK2 Antikörper (ABIN7464217)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CHEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Güteklasse: KO Validated

Klon: 4B8

Produktdetails anti-CHEK2 Antikörper

Kreuzreaktivität: Human

Aufreinigung: Affinity purified by Protein G.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: 293T , A431 , HepG2 , HeLa

Validation: KO/KD

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.85 mg/mL

Buffer: PBS, No Preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Andere Bezeichnung: checkpoint kinase 2

Hintergrund: Checkpoint kinase 2 , CDS1 , CHK2 , HuCds1 , LFS2 , PP1425 , RAD53 , hCds1,In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Molekulargewicht: 61 kDa

Gen-ID: 11200

UniProt: O96017

Pathways: p53 Signalweg, Apoptose, Zellzyklus