Rekombinanter PD-L1 Antikörper (C-Term)

Produktnummer ABIN7455244

Der Kaninchen Monoklonal Anti-PD-L1 Antikörper wurde für WB, FACS, IHC, IP, IF (p), IF/ICC und mIF validiert. Er ist geeignet, PD-L1 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter PD-L1 Antikörper (C-Term) (ABIN7455244)

Target: PD-L1 (CD274 (PD-L1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser PD-L1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (fixed cells) (IF/ICC), Multiplex Immunofluorescence (mIF)

Klon: BLR020E

Produktdetails anti-PD-L1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Rabbit anti-PD-L1 Recombinant Monoclonal Antibody [BLR020E]

Aufreinigung: Purified

Immunogen: between AA 250 to 290 (C-term)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

ICC-IF: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

IHC-IF: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 20 μL/mg lysate

WB: 17:40:00

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 50 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: 2 - 8 °C

Haltbarkeit: 12 months

Details zu PD-L1

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: PD-L1

Hintergrund: PD-L1 is an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. PD-L1 is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma [taken from NCBI Entrez Gene (Gene ID: 55193)].

Gen-ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints