14-3-3 sigma/SFN Antikörper (AA 198-248)

Produktnummer ABIN7454767

Der Kaninchen Polyklonal Anti-14-3-3 sigma/SFN-Antikörper wurde für WB, IP und IHC (fp) validiert. Er ist geeignet, 14-3-3 sigma/SFN in Proben von Human zu detektieren.

Kurzübersicht für 14-3-3 sigma/SFN Antikörper (AA 198-248) (ABIN7454767)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser 14-3-3 sigma/SFN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-14-3-3 sigma/SFN Antikörper

Bindungsspezifität: AA 198-248

Verwendungszweck: Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified

Aufreinigung: Affinity Purified

Immunogen: between AA 198 and 248

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:10,000 - 1:25,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Andere Bezeichnung: 14-3-3 Sigma

Hintergrund: Background: 14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis.

Gen-ID: 2810

NCBI Accession: NP_006133

UniProt: P31947

Pathways: p53 Signalweg, Myometrial Relaxation and Contraction