MCM5 Antikörper (AA 1-50)

Produktnummer ABIN7454594

Der Kaninchen Polyklonal anti-MCM5 Antikörper (ABIN7454594) detektiert spezifisch MCM5 in WB, IP und IHC (fp). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für MCM5 Antikörper (AA 1-50) (ABIN7454594)

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCM5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-MCM5 Antikörper

Bindungsspezifität: AA 1-50

Verwendungszweck: Rabbit anti-MCM5 Antibody, Affinity Purified

Aufreinigung: Affinity Purified

Immunogen: between AA 1 and 50

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 4 μg/mg lysate. This antibody is efficient at pulling down the MCM2-7 complex.

WB: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu MCM5

Target: MCM5 (Minichromosome Maintenance Complex Component 5 (MCM5))

Andere Bezeichnung: MCM5

Hintergrund: Background: The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM5 can form a subcomplex with MCM3. MCM5 also appears to play a role in transcription. MCM5 has been shown to interact with the signal transducer and activator of transcription 1 (STAT1) and demonstrated to be essential for STAT1 target gene transcription.

Gen-ID: 4174

NCBI Accession: NP_006730

UniProt: P33992

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA