Rekombinanter GFP Antikörper (Biotin)

Produktnummer ABIN7272885

Der Alpaca Monoklonal Anti-GFP-Antikörper wurde für IF, ICC und FACS validiert. Er ist geeignet, GFP in Proben von Aequorea victoria und Aequorea aequorea zu detektieren.

Kurzübersicht für Rekombinanter GFP Antikörper (Biotin) (ABIN7272885)

Target: GFP (Green Fluorescent Protein (GFP))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Aequorea victoria, Aequorea aequorea

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser GFP Antikörper ist konjugiert mit Biotin

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Klon: 1H1

Produktdetails anti-GFP Antikörper (Biotin)

Expressionssystem: E.coli

Spezifität: GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pH luorin, paGFP, superfolder GFP, eYFP, mVenus, mClover3 and Citrine

Aufreinigung: Produced in: E.coli

Immunogen: Our sdAbs bind strongly to most of the fluorescent proteins derived from Aequorea Victoria

Anwendungsinformationen

Applikationshinweise: Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconsitute with 250 µL 50% glycerol for storage at -80 °C

Buffer: sdAbs are lyophilized from PBS pH 7.4.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.

Details zu GFP

Target: GFP (Green Fluorescent Protein (GFP))

Andere Bezeichnung: GFP

Hintergrund: A breakthrough in the field of biology and bioluminescence started in the 60s with the discovery of a glowing protein obtained from the jellyfish Aequorea victoria by Osamu Shimomura and colleagues. However, it was only in the early 90s when the green fluorescent protein (GFP) sequence was cloned and used inside a foreign organism as a fluorescent marker. Today more than 800 entries of various fluorescent proteins can be found in an open-source database with the most currently available variants here.