Rekombinanter GFAP Antikörper (Alexa Fluor 647)

Produktnummer ABIN7272867

Der Alpaca Monoklonal Anti-GFAP Antikörper wurde für IHC, IF und ICC validiert. Er ist geeignet, GFAP in Proben von Ratte und Maus zu detektieren.

Kurzübersicht für Rekombinanter GFAP Antikörper (Alexa Fluor 647) (ABIN7272867)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Ratte, Maus

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 1D12

Produktdetails anti-GFAP Antikörper (Alexa Fluor 647)

Expressionssystem: E.coli

Spezifität: Recognizes all isotypes from mouse and rat GFAP. Other species not tested

Aufreinigung: Produced in: E.coli

Immunogen: A fluorescently conjugated single-domain antibody that recognizes mouse and rat GFAP. The single-domain antibody carries two fluorophores and is equipped with specially designed linkers to work in expansion microscopy or enhance post-staining fixation for applications like dSTORM and DNA-PAINT.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Anwendungsinformationen

Applikationshinweise: IF: 1:500 (corresponding to 5 nM final concentration)

Kommentare:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund: Glial fibrillary acidic protein (GFAP) belongs to type III intermediate filaments (like vimentin) and is expressed in various cell types throughout the human body. However, in the central nervous system, it is almost exclusively present in astrocytes, resulting in an important marker to differentiate neurons from astrocytes. GFAP is used primarily as an excellent astrocyte cellular marker.