Nemo-Like Kinase Antikörper

Produktnummer ABIN7268846

Der Kaninchen Monoklonal Anti-Nemo-Like Kinase-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Nemo-Like Kinase in Proben von Human zu detektieren.

Kurzübersicht für Nemo-Like Kinase Antikörper (ABIN7268846)

Target: Nemo-Like Kinase (NLK)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Nemo-Like Kinase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Nemo-Like Kinase Antikörper

Verwendungszweck: NLK Rabbit mAb

Kreuzreaktivität: Human, Maus, Ratte

Produktmerkmale: Monoclonal Antibodies

Aufreinigung: Affinity purification

Immunogen: Recombinant protein of human NLK.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,0.05 % BSA,50 % glycerol, pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu Nemo-Like Kinase

Target: Nemo-Like Kinase (NLK)

Andere Bezeichnung: NLK

Hintergrund: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner.,NLK, nemo like kinase,Epigenetics & Nuclear Signaling,Signal Transduction,Kinase,Serine/threonine kinases,Cell Biology & Developmental Biology,Cell Cycle,Wnt/β-Catenin Signaling Pathway,Stem Cells,NLK

Molekulargewicht: 58kDa

Gen-ID: 51701

UniProt: Q9UBE8

Pathways: Ubiquitin Proteasome Pathway