TRPM2 Antikörper (Extracellular) (FITC)

Produktnummer ABIN7237899

Der FITC-konjugierte Kaninchen Polyklonal anti-TRPM2 Antikörper (ABIN7237899) detektiert spezifisch TRPM2 in FACS und LCI. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für TRPM2 Antikörper (Extracellular) (FITC) (ABIN7237899)

Target: TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRPM2 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-TRPM2 Antikörper (FITC)

Bindungsspezifität: AA 999-1012, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to TRPM2 Channel conjugated to the fluorescent dye FITC

Spezifität: 3rd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human,mouse,rat - 13 out of 14 amino acid residues identical

Produktmerkmale: Extracellular, 3rd loop.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NGTDPYKPK(S)PESD, corresponding to amino acid residues 999-1012 of human TRPM2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Kommentare:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7237447)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 50 μL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu TRPM2

Target: TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))

Andere Bezeichnung: TRPM2

Hintergrund: Transient receptor potential cation channel subfamily M member 2, Transient receptor potential melastatin 2, LTrpC2, TrpC7,The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas1-3. The TRPM family consists of eight members: TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3, TRPM2 and TRPM8, TRPM4 and TRPM5, and TRPM6 and TRPM71,2.The TRPM proteins share structural homology with other members of the TRP superfamily channels, six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family1-3. The C-terminal regions of three TRPM members (TRPM2, TRPM6 and TRPM7) are occupied by enzymatic domains. Almost all the C-terminal region of TRPM2 has an enzymatic domain with sequence similarities to Nudix hydrolases (cleave mononucleotide and dinucleotide polyphosphates2,4.TRPM2 (LTRpC2, TrpC7) is a Ca2+-permeable, nonselective cation channel that is predominantly expressed in various regions of the brain, where it is preferentially localized in microglial cells, the host macrophages of the central nervous system and is also expressed in other tissues, including spleen, heart, liver, lung, and bone marrow. Several splice variants of TRPM2 have been identified5,3.

Alternative names: Transient receptor potential cation channel subfamily M member 2, Transient receptor potential melastatin 2, LTrpC2, TrpC7

Gen-ID: 7226

NCBI Accession: NM_003307

UniProt: O94759