PTAFR Antikörper (Extracellular, Loop 3)

Produktnummer ABIN7887006

Der Kaninchen Polyklonal anti-PTAFR Antikörper wird verwendet zum Nachweis von PTAFR in Proben von Maus. Er wurde validiert für WB, IHC, FACS und LCI.

Kurzübersicht für PTAFR Antikörper (Extracellular, Loop 3) (ABIN7887006)

Target: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PTAFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-PTAFR Antikörper

Bindungsspezifität: AA 259-274, Extracellular, Loop 3

Verwendungszweck: A Rabbit Polyclonal antibody to Platelet-activating factor receptor (extracellular)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat - identical,Human - 13 out of 16 amino acid residues identical

Produktmerkmale: Extracellular, 3rd loop

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)ELGYQTNFHQAINDAH, corresponding to amino acid residues 259 - 274 of mouse PTAFR 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:500

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236256)

Blocking Peptide: (ABIN7236256)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu PTAFR

Target: PTAFR (Platelet-Activating Factor Receptor (PTAFR))

Andere Bezeichnung: PTAFR

Hintergrund:

Synonyms: PTAFR, PAFR

Description: Platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a biologically active phospholipid mediator that was first described by its ability to cause platelet aggregation and dilation of blood vessels. In addition, PAF acts as a mediator of cell-to-cell communication, inflammation, allergic responses and shock1. PAF acts on PAF receptor (PAFR) a G protein-coupled receptor (GPCR). It consists of 7 transmembrane-spanning domains (TMDs), and two potential N-linked glycosylation sites. PAFR was found to expressed in the lung, neutrophils, monocytes, macrophages, placenta, small intestine, heart, liver, kidney, brain, and spleen. As a results of PAF interaction, PAFR is coupled to Gq protein and consequently activates various downstream signaling pathways2. Experiments in both PAF receptor knockout animals and transgenic animals overexpressing PAF receptors support the pathophysiological role attributed to PAF receptor signaling. For example, Mice model of obstructive nephropathy show that PAFR signaling contributes to a pro-inflammatory environment leading to renal dysfunction and progressive organ failure3. In addition, inhibition of PAFR signaling results in an effective inhibition of experimental tumor growth and metastasis4.

Gen-ID: 19204

NCBI Accession: NM_000952

UniProt: Q62035

Pathways: Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin