PINK1 Antikörper (C-Term)

Produktnummer ABIN7233046

Dieser Maus Monoklonal Antikörper detektiert spezifisch PINK1 in WB, IHC und IF/ICC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für PINK1 Antikörper (C-Term) (ABIN7233046)

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PINK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC)

Klon: S4-15

Produktdetails anti-PINK1 Antikörper

Bindungsspezifität: AA 112-496, C-Term

Verwendungszweck: Anti-PINK1 Mouse Monoclonal Antibody

Spezifität: This antibody recognizes human, mouse, and rat PINK1.

Kreuzreaktivität: Human, Maus, Ratte

Immunogen: Fusion protein corresponding to aa 112-496 (cytoplasmic C-terminus) of human PINK1. This sequence is 82

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 1-5 μg/mL. A band of ~50 kDa is detected.
Immunofluorescence: use at 10 μg/mL.
These are recommended concentrations.
Enduser should determine optimal concentrations for their application.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.1 % sodium azide, 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: This product is stable for at least one (1) year at -20°C.

Details zu PINK1

Target: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Andere Bezeichnung: PINK1

Hintergrund: Serine/threonine-protein kinase PINK1, mitochondrial,PINK1 (PTEN-induced putative kinase 1) is a mitochondrial serine/threonine kinase that maintains mitochondrial function and integrity, provides protection against mitochondrial dysfunction during cellular stress, and is involved in the clearance of damaged mitochondria via selective autophagy. PINK1 is synthesized as a 63 kDa protein which undergoes proteolytic processing to yield two cleaved forms, ~55 kDa and ~42 kDa. PINK1 may be localized to the outer mitochondrial membrane with its kinase domain facing the cytosol which would explain the observed physical interaction between PINK1 and cytosolic E3 ubiquitin ligase Parkin.

UniProt: Q9BXM7

Pathways: Autophagie