MLH1 Antikörper

Produktnummer ABIN7232932

Dieser Maus Monoklonal Antikörper detektiert spezifisch MLH1 in IHC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für MLH1 Antikörper (ABIN7232932)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MLH1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: G409-1

Produktdetails anti-MLH1 Antikörper

Verwendungszweck: Anti-MLH1 Mouse Monoclonal Antibody

Spezifität: Human MLH1. Reactivity with other species has not been investigated.

Kreuzreaktivität: Human

Immunogen: Recombinant human MLH1

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:
Immunohistochemistry: use at a dilution of 1:100-1:200 on formalin-fixed, paraffin-embedded samples after heat-induced epitope retrieval at pH 9 for 10-30 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: Lot specific

Buffer: Tris buffer, pH 7.3-7.7, 1 % BSA, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Andere Bezeichnung: MLH1

Hintergrund: DNA mismatch repair protein Mlh1,Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. {PubMed:16873062, PubMed:18206974, PubMed:20020535, PubMed:21120944, PubMed:9311737}.,Nucleus, Chromosome

UniProt: P40692

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response