ROR2 Antikörper (AA 2535-2835)

Produktnummer ABIN7232906

Der Maus Monoklonal Anti-ROR2-Antikörper wurde für WB und IHC validiert. Er ist geeignet, ROR2 in Proben von Maus zu detektieren.

Kurzübersicht für ROR2 Antikörper (AA 2535-2835) (ABIN7232906)

Target: ROR2 (Receptor Tyrosine Kinase-Like Orphan Receptor 2 (ROR2))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ROR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: ROR2 2535-2835

Produktdetails anti-ROR2 Antikörper

Bindungsspezifität: AA 2535-2835

Verwendungszweck: Anti-ROR2 Mouse Monoclonal Antibody

Spezifität: Mouse and human ROR2. Does not cross-react with ROR1.

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified by Protein G affinity chromatography

Immunogen: Bases 2535-2835 of mouse ROR2 fused in-frame to MBP and expressed in E. coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 1-10 μg/mL. A band of ~105 kDa is detected.
Immunohistochemistry: use at 1-10 μg/mL on paraformaldehyde-fixed, paraffin-embedded sections following antigen retrieval (microwave 12 min in citrate buffer, pH 6.0).
These are recommended concentrations.
Enduser should determine optimal concentrations for their application.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium that is identical to that used in the assay system.

Konzentration: Lot specific

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -20°C.

Details zu ROR2

Target: ROR2 (Receptor Tyrosine Kinase-Like Orphan Receptor 2 (ROR2))

Andere Bezeichnung: ROR2

Hintergrund: Tyrosine-protein kinase transmembrane receptor ROR2,Tyrosine-protein kinase receptor which may be involved in the early formation of the chondrocytes. It seems to be required for cartilage and growth plate development (By similarity). Phosphorylates YWHAB, leading to induction of osteogenesis and bone formation (PubMed:17717073). In contrast, has also been shown to have very little tyrosine kinase activity in vitro. May act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PubMed:25029443). {UniProtKB:Q9Z138, PubMed:17717073, PubMed:25029443}.,Receptor tyrosine kinases (RTKs) are cell surface receptors that regulate normal cellular processes through ligand-controlled tyrosine kinase activity. ROR2 is a membrane-bound RTK that is activated by Wnt signaling during normal bone and cartilage development. Recently, ROR2 has been shown to have pro-tumorigenic effects in osteosarcoma, melanoma, and renal cell carcinoma cell lines. ROR2 levels have also been described in soft-tissue sarcomas, specifically, in vitro studies revealed that invasive abilities of leiomyosarcoma (LMS) and gastrointestinal stromal tumor (GIST) are affected by ROR2 expression, and suppression of ROR2 reduces in vivo tumor mass in a xenotransplantation model of LMS.,Cell membrane, Single-pass type I membrane protein,EC 2.7.10.1, Neurotrophic tyrosine kinase, receptor-related 2

UniProt: Q01974

Pathways: RTK Signalweg, WNT Signalweg