BNIP3 Antikörper (C-Term)

Produktnummer ABIN7232776

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BNIP3 in WB und IF/ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für BNIP3 Antikörper (C-Term) (ABIN7232776)

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BNIP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (fixed cells) (IF/ICC)

Produktdetails anti-BNIP3 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-BNIP3 Rabbit Polyclonal Antibody

Spezifität: This antibody recognizes human and mouse BNIP3.

Kreuzreaktivität: Human, Maus

Immunogen: Synthetic peptide corresponding to amino acids at the C-terminus of human BNIP3.

Anwendungsinformationen

Applikationshinweise: Immunoblotting: use at 1 μg/mL. A band of ~30 kDa is detected, an additional ~60 kDa band, complexed BNIP3, may also be detected.
Immunofluorescence: use at 10 μg/mL.
These are recommended concentrations.
Endusers should determine optimal concentrations for their applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: This antibody is stable for at least one (1) year at -20°C.

Details zu BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Andere Bezeichnung: BNIP3

Hintergrund: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3,Apoptosis-inducing protein that can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. Plays an important role in the calprotectin (S100A8/A9)-induced cell death pathway. {PubMed:19935772, PubMed:22292033}.,BNIP3 is a member of the apoptotic Bcl-2 protein family that is involved in both necrosis and apoptosis. Humans and other animals, as well as lower eukaryotes, encode several BNIP3 paralogues including human BNIP3L which induces apoptosis by interacting with viral and cellular anti-apoptosis proteins. BNIP3 interacts with the E1B 19 kDa protein, which is responsible for the protection of virally induced cell death, as well as with E1B-like sequences of BCL2 which is also an apoptotic protector. The BNIP3 gene contains a BH3 domain and a transmembrane domain that have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by the BNIP3 gene is known to induce apoptosis, even in the presence of BCL2.,Mitochondrion. Mitochondrion outer membrane, Single-pass membrane protein. Note=Coexpression with the EIB 19- kDa protein results in a shift in NIP3 localization pattern to the nuclear envelope. Colocalizes with ACAA2 in the mitochondria. Colocalizes with SPATA18 at the mitochondrion outer membrane.

Gen-ID: 664

NCBI Accession: NP_004043

UniProt: Q12983

Pathways: Autophagie, Brown Fat Cell Differentiation