WISP1 Antikörper

Produktnummer ABIN7232144

Der Kaninchen Polyklonal anti-WISP1 Antikörper wird verwendet zum Nachweis von WISP1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und ELISA.

Kurzübersicht für WISP1 Antikörper (ABIN7232144)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WISP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-WISP1 Antikörper

Verwendungszweck: Rabbit Anti-WISP1 Polyclonal Antibody

Spezifität: The antibody detects endogenous levels of WISP1 protein

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human WISP1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu WISP1

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Andere Bezeichnung: WISP1

Hintergrund: WNT1-inducible-signaling pathway protein 1, WISP-1, CCN family member 4, Wnt-1-induced secreted proteinWISP1 encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83 % identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.

Molekulargewicht: 40kD

Gen-ID: 8840

UniProt: O95388

Pathways: WNT Signalweg, Growth Factor Binding