TDG Antikörper (AA 1-80)

Produktnummer ABIN7224638

Der Kaninchen Polyklonal anti-TDG Antikörper wird verwendet zum Nachweis von TDG in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA, IF und IHC.

Kurzübersicht für TDG Antikörper (AA 1-80) (ABIN7224638)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TDG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Produktdetails anti-TDG Antikörper

Bindungsspezifität: AA 1-80

Verwendungszweck: Rabbit Anti-TDG Polyclonal Antibody

Spezifität: TDG Polyclonal Antibody detects endogenous levels of TDG protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human TDG at AA range: 1-80

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Andere Bezeichnung: TDG

Hintergrund: TDG, G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylaseThe protein encoded by TDG (thymine DNA glycosylase) belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. TDG may have a pseudogene in the p arm of chromosome 12.

Molekulargewicht: 46kD

Gen-ID: 6996

UniProt: Q13569

Pathways: DNA Reparatur, Chromatin Binding