MGMT Antikörper (AA 1-80)

Produktnummer ABIN7215758

Der Kaninchen-Polyklonal Anti-MGMT-Antikörper wurde für WB, ELISA und IHC (p) validiert. Er ist geeignet zum Nachweis von MGMT in Proben aus Human und Ratte. }

Kurzübersicht für MGMT Antikörper (AA 1-80) (ABIN7215758)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MGMT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-MGMT Antikörper

Bindungsspezifität: AA 1-80

Verwendungszweck: MGMT Polyclonal Antibody

Spezifität: MGMT Polyclonal Antibody detects endogenous levels of MGMT protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the N-terminal region of human MGMT at AA range: 1-80

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Andere Bezeichnung: MGMT

Hintergrund: Rabbit Anti-MGMT Polyclonal Antibody,MGMT, Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase,Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. Methylated-DNA--protein-cysteine methyltransferase encoded by MGMT is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. Methylated-DNA--protein-cysteine methyltransferase catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.,Methylated-DNA--protein-cysteine methyltransferase

Molekulargewicht: observerd band 22kDa

Gen-ID: 4255

UniProt: P16455

Pathways: DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus