NPM1 Antikörper (pThr199)

Produktnummer ABIN7222808

Der Kaninchen Polyklonal anti-NPM1 Antikörper wird verwendet zum Nachweis von NPM1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF und ELISA.

Kurzübersicht für NPM1 Antikörper (pThr199) (ABIN7222808)

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NPM1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Produktdetails anti-NPM1 Antikörper

Bindungsspezifität: pThr199

Verwendungszweck: Rabbit Anti-B23 (phospho Thr199) Polyclonal Antibody

Spezifität: Phospho-B23 (T199) Polyclonal Antibody detects endogenous levels of B23 protein only when phosphorylated at T199.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human B23 Phospho-Thr199

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Andere Bezeichnung: B23

Hintergrund: NPM1, NPM, Nucleophosmin, NPM, Nucleolar phosphoprotein B23, Nucleolar protein NO38, NumatrinNPM1 encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of NPM1 have been identified. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 32kD

Gen-ID: 4869

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly