HNF4A Antikörper (pSer313)

Produktnummer ABIN7213334

Der Kaninchen Polyklonal Anti-HNF4A Antikörper wurde für WB, ELISA, IF und IHC (p) validiert. Er ist geeignet, HNF4A in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für HNF4A Antikörper (pSer313) (ABIN7213334)

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HNF4A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HNF4A Antikörper

Bindungsspezifität: pSer313

Verwendungszweck: HNF4-α (phospho Ser313) Polyclonal Antibody

Spezifität: Phospho-HNF4-α (S313) Polyclonal Antibody detects endogenous levels of HNF4-α protein only when phosphorylated at S313.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human HNF4-alpha Phospho-Ser313

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu HNF4A

Target: HNF4A (Hepatocyte Nuclear Factor 4, alpha (HNF4A))

Andere Bezeichnung: HNF4-alpha

Hintergrund: Rabbit Anti-HNF4-α (phospho Ser313) Polyclonal Antibody,HNF4A, HNF4, NR2A1, TCF14, Hepatocyte nuclear factor 4-alpha, HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4,Hepatocyte nuclear factor 4 alpha encoded by HNF4A is a nuclear transcription factor which binds DNA as a homodimer. Hepatocyte nuclear factor 4 alpha controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of HNF4A results in multiple transcript variants encoding several different isoforms.,Hepatocyte nuclear factor 4-alpha

Molekulargewicht: observerd band 52kDa

Gen-ID: 3172

UniProt: P41235

Pathways: AMPK Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process