FEN1 Antikörper (AA 60-140)

Produktnummer ABIN7214884

Der Kaninchen Polyklonal anti-FEN1 Antikörper (ABIN7214884) detektiert spezifisch FEN1 in WB, IHC, IF und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für FEN1 Antikörper (AA 60-140) (ABIN7214884)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FEN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Produktdetails anti-FEN1 Antikörper

Bindungsspezifität: AA 60-140

Verwendungszweck: Rabbit Anti-FEN-1 Polyclonal Antibody

Spezifität: FEN-1 Polyclonal Antibody detects endogenous levels of FEN-1 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human FEN-1 at AA range: 60-140

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Andere Bezeichnung: FEN-1

Hintergrund: FEN1, RAD2, Flap endonuclease 1, FEN-1, DNase IV, Flap structure-specific endonuclease 1, Maturation factor 1, MF1, hFEN-1Flap endonuclease 1 encoded by FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.

Molekulargewicht: 42kD

Gen-ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA