Cyclin D2 Antikörper (pThr280)

Produktnummer ABIN7225726

Der Kaninchen Polyklonal anti-Cyclin D2 Antikörper wird verwendet zum Nachweis von Cyclin D2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, ELISA und IHC (p).

Kurzübersicht für Cyclin D2 Antikörper (pThr280) (ABIN7225726)

Target: Cyclin D2 (CCND2)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin D2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Cyclin D2 Antikörper

Bindungsspezifität: pThr280

Verwendungszweck: Cyclin D2 (phospho Thr280) Polyclonal Antibody

Spezifität: Phospho-Cyclin D2 (T280) Polyclonal Antibody detects endogenous levels of Cyclin D2 protein only when phosphorylated at T280.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cyclin D2 Phospho-Thr280

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Kommentare:

Primary Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Details zu Cyclin D2

Target: Cyclin D2 (CCND2)

Andere Bezeichnung: Cyclin D2

Hintergrund: Rabbit Anti-Cyclin D2 (phospho Thr280) Polyclonal Antibody,CCND2, G1/S-specific cyclin-D2,The protein encoded by CCND2 (cyclin D2) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK4 or CDK6 and functions as a regulatory subunit of the complex, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. Knockout studies of the homologous gene in mouse suggest the essential roles of this gene in ovarian granulosa and germ cell proliferation. High level expression of CCND2 was observed in ovarian and testicular tumors. Mutations in CCND2 are associated with megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 3 (MPPH3).,cyclin-D2

Molekulargewicht: observerd band 40kDa

Gen-ID: 894

UniProt: P30279

Pathways: Zellzyklus, Mitotic G1-G1/S Phases