CDC25A Antikörper

Produktnummer ABIN7226162

Der Kaninchen Polyklonal anti-CDC25A Antikörper (ABIN7226162) detektiert spezifisch CDC25A in WB, ELISA, IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Affe.

Kurzübersicht für CDC25A Antikörper (ABIN7226162)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC25A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-CDC25A Antikörper

Verwendungszweck: Rabbit Anti-Cdc25A Polyclonal Antibody

Spezifität: Cdc25A Polyclonal Antibody detects endogenous levels of Cdc25A protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cdc25A around the non-phosphorylation site of S75

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Andere Bezeichnung: Cdc25A

Hintergrund: CDC25A, M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25ACDC25A (cell division cycle 25A) is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 60kD

Gen-ID: 993

UniProt: P30304

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase