CD72 Antikörper (Internal Region)

Produktnummer ABIN7226672

Der Kaninchen Polyklonal anti-CD72 Antikörper (ABIN7226672) detektiert spezifisch CD72 in WB, IHC, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für CD72 Antikörper (Internal Region) (ABIN7226672)

Target: CD72 (CD72 Molecule (CD72))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD72 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-CD72 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Rabbit Anti-CD72 Polyclonal Antibody

Spezifität: CD72 Polyclonal Antibody detects endogenous levels of CD72 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CD72

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-1:2000,ELISA 1:10000,IF 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CD72

Target: CD72 (CD72 Molecule (CD72))

Andere Bezeichnung: CD72

Hintergrund: CD72, B-cell differentiation antigen CD72, Lyb-2, CD72CD5 has been identified as a transmembrane glycoprotein that is expressed on 70 % of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/CD3 ζ chain, and with the Src family kinase, Lck p56. The C-type lectin superfamily member CD72 is a cell surface negative regulator of B cell activation from the pro-B through the mature B cell stage. CD72 serves as a receptor for CD5. The ability of lymphocytes to respond to antigenic or mitogenic stimulation utilizes both positive and negative regulatory proteins that influence the threshold for responsiveness. The human CD72 gene maps to chromosome 9p13.3 and encodes a transmembrane glycoprotein that contains an immunoreceptor tyrosine-based inhibition motif (ITIM). Upon tyrosine phosphorylation, the CD72 ITIM recruits SH2-containing phosphatases such as SHP-1, resulting in downregulation of cell activation. CD72-/- mice contain hyperproliferative B cells.

Molekulargewicht: 40kD

Gen-ID: 971

UniProt: P21854

Pathways: BCR Signaling