MSH2 Antikörper

Produktnummer ABIN7183857

Der Kaninchen Polyklonal Anti-MSH2-Antikörper wurde für WB, ELISA, IHC und IF validiert. Er ist geeignet, MSH2 in Proben von Human zu detektieren.

Kurzübersicht für MSH2 Antikörper (ABIN7183857)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MSH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-MSH2 Antikörper

Kreuzreaktivität: Human, Maus

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from internal of Human MSH2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:3000, IHC:1:50-1:200, IF:1:100-1:200,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Andere Bezeichnung: MSH2

Hintergrund:

Background: Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Fishel R., Cell 75:1027-1038(1993).
Wang Y., Genes Dev. 14:927-939(2000).
Wang Y., Proc. Natl. Acad. Sci. U.S.A. 100:15387-15392(2003).

Aliases: BAT26 antibody, COCA 1 antibody, COCA1 antibody, DNA mismatch repair protein Msh2 antibody, FCC 1 antibody, FCC1 antibody, hMSH2 antibody, HNPCC 1 antibody, HNPCC antibody, HNPCC1 antibody, LCFS2 antibody, MSH 2 antibody, Msh2 antibody, MSH2_HUMAN antibody, MutS homolog 2 antibody, MutS homolog 2 colon cancer nonpolyposis type 1 antibody, MutS protein homolog 2 antibody

UniProt: P43246

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response