SMAD3 Antikörper (pSer208)

Produktnummer ABIN712226

Der Kaninchen Polyklonal Anti-SMAD3-Antikörper wurde für WB, ELISA, IHC (p), FACS, ICC, IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, SMAD3 in Proben von Human, Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für SMAD3 Antikörper (pSer208) (ABIN712226)

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-SMAD3 Antikörper

Bindungsspezifität: pSer208

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser208 [NL(p-S)PN]

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-SMAD3 Antikörper (ABIN712226)

Zhang, Ju, Zhang, Zhu, Nie, Xu, Lei et al.: "Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4(+) T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 ..." in: Basic & clinical pharmacology & toxicology, Vol. 120, Issue 6, pp. 560-570, (2016) (PubMed).

Details zu SMAD3

Target: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Andere Bezeichnung: Smad3

Hintergrund:

Synonyms: SMAD3phospho S208, SMAD3phospho Ser208, p-SMAD3Ser208, hMAD 3, hSMAD3, HSPC193, JV15 2, JV152, MAD mothers against decapentaplegic Drosophila homolog 3, MAD3, MADH 3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA and MAD related protein 3, SMAD 3, SMAD, SMAD-3, SMAD3_HUMAN.

Background: Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8), the common partner, (Smad4) that functions via its interaction to the various Smads, and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.

Gen-ID: 4088

Pathways: Zellzyklus, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagie