PKM Antikörper

Produktnummer ABIN7163482

Dieses Anti-PKM-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PKM in ELISA, WB und IP. Geeignet für Human.

Kurzübersicht für PKM Antikörper (ABIN7163482)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PKM Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-PKM Antikörper

Kreuzreaktivität: Human

Aufreinigung: Affinity purified

Immunogen: Recombinant human PKM2 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended dilution:WB:1:500-1:5000,IP:1:200-1:2000,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 1.0 mg/mL in phosphate buffer without Mg2+/Ca2+, pH 7.4, 150 mM NaCl and 50 % glycerol

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Andere Bezeichnung: PKM

Hintergrund:

Background: Pyruvate kinase (PK) regulates the final rate-limiting step of glycolysis in the production of pyruvate and adenosine triphosphate (ATP). Alternate splicing of PKM pre-mRNA leads to PKM2 generation by the inclusion of exon 10 and the exclusion of exon 9, which is specific for PKM1. Besides its cytosolic roles in glycolysis, PKM2, which is upregulated by growth factor receptor activation 1, is phosphorylated at S37 by extracellular signal-regulated kinase (ERK) 2. This phosphorylation leads to the cis-trans isomerization of PKM2 by the peptidyl-prolineisomerase protein interacting with never in mitosis A 1 (PIN1), exposure of the nuclear localization signal (NLS) of PKM2, and subsequent binding of importin α 5 for nuclear translocation 2. In the nucleus, PKM2binds to phosphorylated Y333 of β-catenin, which is essential for β-catenin transactivation 3, and interacts with and phosphorylates histone H3 at T11, leading to H3-K9 acetylation and transcription of genes such as MYC and CCND14. c-Myc expression results in the upregulation of GLUT1, lactate dehydrogenase A (LDHA), and, in a positive feedback loop, PTB-dependent PKM2, which subsequently enhances the Warburg effect 2. Cyclin D1 expression, in turn, promotes G1-S phase transition 3, 4.1 Yang W, Xia Y, Cao Y et al. EGFR-induced and PKCepsilonmonoubiquitylation-dependent NF-kappaB activation upregulates PKM2 expression and promotes tumorigenesis. Molecular cell 2012; 48:771-784.2 Yang W, Zheng Y, Xia Y et al. ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect. Nature cell biology 2012; 14:1295-1304.3 Yang W, Xia Y, Ji H et al. Nuclear PKM2 regulates beta-catenin transactivation upon EGFR activation. Nature 2011; 480:118-122.4 Yang W, Xia Y, Hawke D et al. PKM2 phosphorylates histone H3 and promotes gene transcription and tumorigenesis. Cell 2012; 150:685-696.

Aliases: PKM; PK3; OIP3; PK2;

UniProt: P14618

Pathways: Warburg Effekt