MLH1 Antikörper

Produktnummer ABIN7140335

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MLH1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MLH1 Antikörper (ABIN7140335)

Target: MLH1 (MutL Homolog 1 (MLH1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MLH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-MLH1 Antikörper

Kreuzreaktivität: Human

Aufreinigung: Affinity purified

Immunogen: Purified recombinant human MLH1 protein fragments expressed in E.coli.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Recommended dilution:WB:1:500-1:5000,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified mouse monoclonal in PBS( pH 7.4) containing with 0.02 % sodium azide,0.1 mg/mLBSA and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu MLH1

Target: MLH1 (MutL Homolog 1 (MLH1))

Andere Bezeichnung: MLH1

Hintergrund:

Background: Heterodimerizes with PMS2 to form MutL alpha,a component of the post-replicative DNA mismatch repair system (MMR).DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch,then MutL alpha is recruited to the heteroduplex.Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch.DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected.MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III,suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR.Also implicated in DNA damage signaling,a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages.Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

Aliases: COCA 2, COCA2, DNA mismatch repair protein Mlh1, FCC 2, FCC2, hMLH 1, hMLH1, HNPCC 2, HNPCC, HNPCC2, MGC5172, MLH 1, MLH1, MLH1_HUMAN, MutL homolog 1 (E. coli), MutL homolog 1, MutL homolog 1 colon cancer nonpolyposis type 2,

UniProt: P40692

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response