IRF3 Antikörper (pSer386)

Produktnummer ABIN7138590

Der Kaninchen Polyklonal anti-IRF3 Antikörper wird verwendet zum Nachweis von IRF3 in Proben von Human. Er wurde validiert für WB und ELISA.

Kurzübersicht für IRF3 Antikörper (pSer386) (ABIN7138590)

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-IRF3 Antikörper

Bindungsspezifität: pSer386

Verwendungszweck: Phospho-IRF3 (Ser386) Antibody

Aufreinigung: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of Serine 386(A-S-S(p)-L-E) derived from Human IRF-3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu IRF3

Target: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Andere Bezeichnung: IRF3

Hintergrund:

Background:

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Au W.W.-C., Proc. Natl. Acad. Sci. U.S.A. 92:11657-11661(1995).
The MGC Project Team, Genome Res. 14:2121-2127(2004).
Bellingham J., Ann. Hum. Genet. 62:231-234(1998).

Aliases: IIAE7 antibody, Interferon regulatory factor 3 antibody, IRF 3 antibody, IRF-3 antibody, IRF3 antibody, IRF3_HUMAN antibody, MGC94729 antibody

UniProt: Q14653

Pathways: TLR Signalweg, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades