Rekombinanter FEN1 Antikörper

Produktnummer ABIN7127487

Der Kaninchen-Monoklonal Anti-FEN1-Antikörper wurde für WB, IHC, ELISA und IF validiert. Er ist geeignet zum Nachweis von FEN1 in Proben aus Human. }

Kurzübersicht für Rekombinanter FEN1 Antikörper (ABIN7127487)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser FEN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Klon: 4D9

Produktdetails anti-FEN1 Antikörper

Verwendungszweck: FEN1 Recombinant Monoclonal Antibody

Aufreinigung: Affinity-chromatography

Immunogen: A synthesized peptide derived from human FEN1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Andere Bezeichnung: FEN1

Hintergrund:

Background: Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

Aliases: Flap endonuclease 1UniRule annotation, DNase IV, Flap structure-specific endonuclease 1UniRule annotation, FEN1

UniProt: P39748

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA