Rekombinanter Cytokeratin 2 Antikörper (AA 300-400)

Produktnummer ABIN7127260

Der Kaninchen Monoklonal Anti-Cytokeratin 2-Antikörper wurde für IHC (f) validiert. Er ist geeignet, Cytokeratin 2 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Cytokeratin 2 Antikörper (AA 300-400) (ABIN7127260)

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Cytokeratin 2 Antikörper

Bindungsspezifität: AA 300-400

Spezifität: The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to Type II Cytokeratins residues within aa300-400 of Type II Cytokeratins

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Skin or squamous cell carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Andere Bezeichnung: KRT76

Hintergrund: KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Type II (Epithelial Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 66/65/64/57/62/60/51/54kDa

Gen-ID: 51350, 654392

UniProt: Q01546