Rekombinanter Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN7127009

Der Maus Monoklonal anti-Topoisomerase II alpha Antikörper (Klon rTOP2A-6569) (ABIN7127009) detektiert spezifisch Topoisomerase II alpha in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für Rekombinanter Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN7127009)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: RTOP2A-6569

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Verwendungszweck: Recombinant Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) Antibody

Spezifität: It recognizes a 170kDa protein, which is identified as topoisomerase II°™. It shows no cross-reaction with Topoisomerase II æ or I. Topo II °™ plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II °™ is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.

Kreuzreaktivität (Details): Human. Mouse.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells. Human lymph node or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A

Hintergrund:

ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170 kDa, TP2A

HeLa or Jurkat cells. Human lymph node or tonsil. A549.

Molekulargewicht: 170kDa

Gen-ID: 7153, 156346

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases