STAT5A Antikörper

Produktnummer ABIN7126986

Dieser Maus Monoklonal Antikörper detektiert spezifisch STAT5A in IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für STAT5A Antikörper (ABIN7126986)

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser STAT5A Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-STAT5A Antikörper

Spezifität: Signal transducer and activator of transcription 5A (Stat5a) and Stat5b, which share 96 % homology, undergo receptor tyrosine kinase or G protein-coupled receptor-dependent phosphorylation in response to cytokines or growth factors, and then form homo- or heterodimers that translocate to the nucleus, where they initiate transcription. Activation of Stat5a via IL-2, IL-3, IL-7/ GM-CSF, erythropoietin, thrombopoietin and growth hormones influences proliferation, differentiation and apoptosis in lymphohematopoietic cells. Phosphorylation of Stat5a at Ser127/Ser128 and Ser779 are contigent on ErbB-4- mediated activation of Stat5a. Activation of Stat5b via IL-2, IL-4, CSF-1 and growth hormones influences TCR signaling, apoptosis, adult mammary gland development and sexual dimorphism of liver gene expression. Stat5b is the major liver-expressed Stat5 form that has been shown to fuse with the retinoic acid receptor a gene in acute promyelocytic leukemias (APLL). Stat5a/b null mice have severely impaired lymphoid development and differentiation.

Kreuzreaktivität (Details): Human. Predicted to work with Mouse and Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Protein domain of STAT5A

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or Jurkat cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu STAT5A

Target: STAT5A (Signal Transducer and Activator of Transcription 5A (STAT5A))

Andere Bezeichnung: STAT5A

Hintergrund: Signal transducer and activator of transcription 5A, Transcription factor STAT5A, Mammary gland factor (MGF),STAT5A
Cellular localisation: Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.

Molekulargewicht: 64kDa

Gen-ID: 6776, 437058

UniProt: P42229

Pathways: JAK-STAT Signalweg, RTK Signalweg, Response to Growth Hormone Stimulus, C21-Steroid Hormone Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events, Activated T Cell Proliferation