Rekombinanter Smooth Muscle Actin Antikörper

Produktnummer ABIN7126869

Der Kaninchen Monoklonal anti-Smooth Muscle Actin Antikörper wird verwendet zum Nachweis von Smooth Muscle Actin in Proben von Human, Ratte, Maus, Kaninchen und Meerschweinchen. Er wurde validiert für WB und IHC.

Kurzübersicht für Rekombinanter Smooth Muscle Actin Antikörper (ABIN7126869)

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus, Kaninchen, Meerschweinchen

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Smooth Muscle Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Klon: ACTA2-1614R

Produktdetails anti-Smooth Muscle Actin Antikörper

Verwendungszweck: Recombinant Actin, Smooth Muscle (Leiomyosarcoma Marker) Antibody

Spezifität: Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human ACTA2 protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Smooth Muscle Actin

Target: Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))

Andere Bezeichnung: ACTA2

Hintergrund:

ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha Cardiac Actin, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46

Kidney, skeletal muscle, Human uterus or leiomyosarcoma, Mouse NIH/3T3 cells.

Molekulargewicht: 42kDa

Gen-ID: 59, 500483

UniProt: P62736

Pathways: Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development