FASL Antikörper (AA 107-222)

Produktnummer ABIN7126570

Der Maus Monoklonal anti-FASL Antikörper (Klon FASLG-4453) (ABIN7126570) detektiert spezifisch FASL in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für FASL Antikörper (AA 107-222) (ABIN7126570)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FASL Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: FASLG-4453

Produktdetails anti-FASL Antikörper

Bindungsspezifität: AA 107-222

Verwendungszweck: Fas Ligand (FASLG) Antibody

Spezifität: Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an importantcomponent of specific effector mechanisms in immuno-surveillance againstvirus-infected or transformed cells. Two mechanisms appear to account forthis activity, one of which is the perforin-based process. Independently, aFAS-based mechanism involves the transducing molecule FAS (also designated Apo-1) and its ligand (FAS-L). The human FAS protein is a cell surfaceglycoprotein that belongs to a family of receptors that includes CD40, nervegrowth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of whichundergo apoptosis in response to treatment with antibody to FAS. Thesefindings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FASmay be critically involved in CTL-mediated cytotoxicity.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human recombinant FASLG protein fragment (around aa107-222) (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil or prostate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Andere Bezeichnung: FASLG

Hintergrund:

ALPS1B, Apoptosis (APO 1) antigen ligand 1, APT1LG1, CD178 antigen, CD95 ligand protein,Generalized lymphoproliferative disease (Gld), soluble form, Tumor necrosis factor ligand superfamily member 6 (TNFL6 / TNFSF6)

Human tonsil or prostate.

Molekulargewicht: 26kDa (soluble FAS-L) / 40kDa (FAS-L membrane)

Gen-ID: 356, 2007

UniProt: P48023

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity