IGF1R Antikörper (AA 550-750)

Produktnummer ABIN7126548

Der Maus Monoklonal Anti-IGF1R-Antikörper wurde für IF, FACS, Neut, AcA und in vitro validiert. Er ist geeignet, IGF1R in Proben von Human zu detektieren.

Kurzübersicht für IGF1R Antikörper (AA 550-750) (ABIN7126548)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IGF1R Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Neutralization (Neut), Activity Assay (AcA), In vitro Assay (in vitro)

Produktdetails anti-IGF1R Antikörper

Bindungsspezifität: AA 550-750

Spezifität: Receptor tyrosine kinases (RTKs) are transmembrane molecular scaffolds that influence cellular processes including the cell cycle, cell migration, cell metabolism, cell survival, proliferation and differentiation. Insulin-like growth factor-I receptor (IGF-IR) is an RTK that stimulates growth in many different cell types, blocks apoptosis, acts as an intermediate of many growth hormone responses and may stimulate the growth of some types of cancer. The IGF-IR cognate ligand Insulin-like growth factor-I (IGF-I) promotes association of IGF-IR with Shc, GRB2 and Sos 1, which initiates Ras and ERK kinase cascades, thereby modifying transcription factor activity, such as activation of the Elk transcription factors. The modular phosphotyrosine binding (PTB) domains of Insulin receptor substrate (IRS)-1 and -2 can associate with active IGF-IR and initiate phosphatidylinositol 3-kinase-dependent downstream signals. The human IGF-IR gene maps to chromosome 15q26.3 and encodes a 1,376 amino acid precursor protein that cleaves into i± and i² subunits. The human IGF-IIR gene maps to chromosome 6q26 and encodes a 2,491 amino acid transmembrane protein.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa550-750) of human IGF1R protein (exact sequence is proprietary)

Isotyp: IgG2

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-4 μg/mL), In-vitro Neutralization of Biological Activity of IGF-1 (Order Ab without Azide)Optimal dilution for a specific application should be determined.

Positive_Control: Human breast carcinoma or colon carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Andere Bezeichnung: IGF1R

Hintergrund: CD221 antigen, IGF 1 receptor, IGF 1R, IGFIR, IGFIRC, IGFR, Insulin like growth factor 1 receptor precursor, Insulin-like growth factor 1 receptor beta chain, JTK13, Soluble IGF1R variant 1, Soluble IGF1R variant 2,IGF1R / CD221
Cellular localisation: Cell membrane.

Molekulargewicht: 130kDa (IGF-1R), 97kDa (IGF-1R), 200kDa (pro-IGF-1R)

Gen-ID: 3480, 643120, 714012

UniProt: P08069

Pathways: RTK Signalweg, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagie