Rekombinanter IDH1 Antikörper (AA 281-414)

Produktnummer ABIN7126537

Dieses Anti-IDH1-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von IDH1 in IHC (f). Geeignet für Human.

Kurzübersicht für Rekombinanter IDH1 Antikörper (AA 281-414) (ABIN7126537)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser IDH1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-IDH1 Antikörper

Bindungsspezifität: AA 281-414

Spezifität: IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human IDH1 protein (around aa 281-414) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa, HT29 or MCF7cells. Humanbreast, colon or prostate carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Andere Bezeichnung: IDH1

Hintergrund: Cytosolic NADP-isocitrate dehydrogenase, Epididymis luminal protein 216, Epididymis secretory protein Li 26, HEL-216, HEL-S-26, ICDH, IDCD, IDH1, IDP, IDPC, Isocitrate dehydrogenase 1 (NADP+) soluble, NADP dependent isocitrate dehydrogenase peroxisomal, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD,IDH1-R132H (Isocitrate Dehydrogenase)
Cellular localisation: Cytoplasm. Nucleus.

Molekulargewicht: 45-47kDa

Gen-ID: 3417, 593422

UniProt: O75874

Pathways: Warburg Effekt