Fibronectin 1 Antikörper

Produktnummer ABIN7126426

Dieses Anti-Fibronectin 1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Fibronectin 1 in IF, FACS und IHC (f). Geeignet für Human.

Kurzübersicht für Fibronectin 1 Antikörper (ABIN7126426)

Target: Fibronectin 1 (FN1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibronectin 1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Produktdetails anti-Fibronectin 1 Antikörper

Spezifität: Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 29 kDa N-terminal fibrin- and heparin-binding domain of fibronectin. It does not cross-react with MMP-2, MMP-9, or TIMP-2. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.

Kreuzreaktivität (Details): Human and Pig.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: High molecular weight proteins secreted by cultivated human fibroblasts

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: SW156 cells. Prostate or Kidney.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Fibronectin 1

Target: Fibronectin 1 (FN1)

Andere Bezeichnung: FN1

Hintergrund: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3,Fibronectin (Fibrin/Heparin-Binding Domain)
Cellular localisation: Connective tissue matrix

Molekulargewicht: 220kDa (monomer), 440kDa (dimer)

Gen-ID: 2335, 203717

UniProt: P02751

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagie