Rekombinanter CTNNB1 Antikörper

Produktnummer ABIN7126268

Dieser Maus Monoklonal Antikörper erkennt spezifisch CTNNB1 in ELISA, FACS, IHC (f) und Coat. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter CTNNB1 Antikörper (ABIN7126268)

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CTNNB1 Antikörper ist unkonjugiert

Applikation: ELISA, Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Produktdetails anti-CTNNB1 Antikörper

Spezifität: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

Kreuzreaktivität (Details): Human, Mouse and Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human beta-Catenin (p120) protein fragment

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), ELISA (For coating, order antibody without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or MCF-7 cells. Liver tissue lysate.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CTNNB1

Target: CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))

Andere Bezeichnung: CTNNB1

Hintergrund: Cadherin associated protein, beta 1 88 kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1,Catenin, beta (p120)
Cellular localisation: Cell surface and cytoplasmic

Molekulargewicht: 92kDa

Gen-ID: 1499, 476018

UniProt: P35222

Pathways: WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2