CHEK2 Antikörper

Produktnummer ABIN7126216

Dieses Anti-CHEK2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CHEK2 in WB, IF, IP und FACS. Geeignet für Human.

Kurzübersicht für CHEK2 Antikörper (ABIN7126216)

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CHEK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS)

Produktdetails anti-CHEK2 Antikörper

Spezifität: Recognizes a serine/threonine protein kinase that is a required check-point to mediate cell cycle arrest, activation of DNA repair and apoptosis. In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. Also, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors.

Kreuzreaktivität (Details): Human. Predicted to react with Mouse and Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human CHEK2 corresponding to protein domain

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), ,Western Blot (1-2 μg/mL),Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HCT 116 cells. Human testis, spleen or colon.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CHEK2

Target: CHEK2 (Checkpoint Kinase 2 (CHEK2))

Andere Bezeichnung: CHEK2

Hintergrund: Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHK2 checkpoint homolog (S. pombe), hCds1, HuCds 1, LFS2, PP1425, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2,CHEK2
Cellular localisation: Nucleus.

Molekulargewicht: 60.91kDa

Gen-ID: 11200, 291363, 505297

UniProt: O96017

Pathways: p53 Signalweg, Apoptose, Zellzyklus