MSH6 Antikörper

Produktnummer ABIN7116545

Der Maus Monoklonal Anti-MSH6-Antikörper wurde für WB, IHC, ELISA und IP validiert. Er ist geeignet, MSH6 in Proben von Human zu detektieren.

Kurzübersicht für MSH6 Antikörper (ABIN7116545)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Klon: 4C4

Produktdetails anti-MSH6 Antikörper

Aufreinigung: Protein A+G purification

Reinheit: ≥95 % as determined by SDS-PAGE

Immunogen: mutS homolog 6

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:2000, IP: 1:500-1:1000, IHC: 1:50-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Haltbarkeit: 12 months

Details zu MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Andere Bezeichnung: MSH6

Hintergrund: Synonyms:G/T mismatch binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, MutS alpha 160 kDa subunit, mutS homolog 6 (E. coli), p160 Background:Component of the post-replicative DNA mismatch repair system(MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops(IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3(H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Molekulargewicht: 160 kDa

Gen-ID: 2956

UniProt: P52701

Pathways: DNA Reparatur, Chromatin Binding, Production of Molecular Mediator of Immune Response