Rekombinanter Integrin beta 2 Antikörper

Produktnummer ABIN7072727

Der Kaninchen Chimeric Anti-Integrin beta 2-Antikörper wurde für WB, ELISA, BR und InhA validiert. Er ist geeignet, Integrin beta 2 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Integrin beta 2 Antikörper (ABIN7072727)

Target: Integrin beta 2 (ITGB2)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser Integrin beta 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Blocking Reagent (BR), Inhibition Assay (InhA)

Klon: 60-3

Produktdetails anti-Integrin beta 2 Antikörper

Verwendungszweck: Anti-CD18 [60.3], Rabbit IgG, kappa

Spezifität: This antibody binds the CD18 component of the β-2 integrin family which is expressed by leukocytes and neutrophils.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with alloantigen activated T cells.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody is capable of inhibiting several cell mediated immune functions. It inhibits proliferative responses of T cells stimulated by soluble antigens, mitogens, or allogeneic cells in the initiation of a culture, but not when added after 48 hours. It successfully blocks the lytic activity of cytotoxic T cells and natural killer cells and the migration of neutrophilic granulocytes (PMID: 6644024). This antibody inhibits phagocytosis by reaction with the C3bi receptor, and stimulates phagocytosis by reverse opsonization (PMID: 3880787). This antibody inhibits aggregation of neutrophils in response phorbol myristate acetate, zymosan-activated plasma, and N-formyl-methionylleucylphenylalanine in a dose dependent manner (PMID: 3995185). 60.3 also inhibits polymorphonuclear leukocyte accumulation and plasma leakage in vivo (PMID: 3539230). 60.3 successfully inhibits neutrophil function in vivo and has the potential to be used as an anti-inflammatory agent (PMID: 3693901). This antibody has application in blocking neutrophil mediated organ damage which occurs after myocardial infarct or hemorrhagic shock. Humanized version of this antibody binds CD18 with a 4-fold less affinity than its parent murine version (PMID: 7937713).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Andere Bezeichnung: CD18

Hintergrund: ITGB2, MFI7, Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta, Complement receptor C3 subunit beta, β2-subunit of leukocyte integrins

UniProt: P05107

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation