Rekombinanter CD74 Antikörper

Produktnummer ABIN7072683

Dieses Anti-CD74-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD74 in FACS, WB, IP und RIA. Geeignet für Human.

Kurzübersicht für Rekombinanter CD74 Antikörper (ABIN7072683)

Target: CD74 (HLA-DR-gamma (CD74))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD74 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Klon: LL1 (EPB1)

Produktdetails anti-CD74 Antikörper

Verwendungszweck: Anti-CD74 [LL1 (EPB1)], Rabbit IgG, kappa

Spezifität: This antibody reacts with the cell surface epitope of the invariant chain (Ii) subunit of the immatrue MHC class-II antigen CD74.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was generated by immunizing female BALB/c mice with whole Raji cells or a crude membrane preparation.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Radioimmunobinding and flow cytometric analysis was used to analyse binding of LL1 to peripheral blood. LL1 did not react with T cell lines nor with normal diploid cells. The reactivity of LL1 (EB-1) to the tumor cells was checked using a live cell indirect immunoflurescence staining assay. LL1 stained positive for cell lines Raji (maxilla), Daudi (B-lymphoblast), RAMOS (b-lymphocyte), SU-DHL-7, ARH-77 (plasma cell leukemia), HS-Sultan (plasmacytoma). LL1 is reported to recognize most of the small and large lymphocytes of the germinal centre and no reactivity to marginal zone B-cells. It is also reported to react with interdigitating histiocytes of the T-cell zones. It was further reported that LL1 bound positively to Western blotted Raji cell antigens (PMID: 2663143). The specificity of LL1 for Ii subunit was determined by immunoprecipitation. Radiolabelled antibodies were used for internalization and catabolism of the antibody and it was seen that LL1 was internalized and catabolized very quickly as compared to some other antibodies like those which are specific for CD19, CD22 and anti-transferrin receptor. (PMID: 8947500). LL1 was evaluated to be a potential candidate for bone marrow imaging using Tcm labelled LL1 sulphur colloid imaging. (PMID: 9076770)

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD74

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74

Hintergrund: Ii, p33, DHLAG, HLADG, Ia-GAMMAHLA, HLA Class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain,

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints