Rekombinanter CD74 Antikörper

Produktnummer ABIN7072640

Der Kaninchen Chimeric Anti-CD74-Antikörper wurde für FACS, WB, IF, IHC, ELISA und IP validiert. Er ist geeignet, CD74 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Rekombinanter CD74 Antikörper (ABIN7072640)

Target: CD74 (HLA-DR-gamma (CD74))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD74 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Klon: LN-2

Produktdetails anti-CD74 Antikörper

Verwendungszweck: Anti-CD74 [LN-2], Rabbit IgG, kappa

Spezifität: LN-2 antibody recognizes CD74. It reacts with an epitope residing within 60 amino acids of the extracytoplasmic, COOH terminus of the protein. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides.

Kreuzreaktivität: Maus, Ratte

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with SU-DHL-4 lymphoma cells.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). This antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells. Furthermore, this antibody was shown to be capable of distinguishing small cell lung cancer from a non small cell lung cancer as a part of a panel of antibodies (Ioachim et al., 1996). Furthermore, LN2 was successfully used in immunohistochemical and flow cytometric analysis of lung cancer tissue and cells (Ioachim et al., 1996). Finally, this antibody is also useful in immunofluorescence analysis of CD74, as it was very effective in labelling this protein in CD74-transfected COS-7 cells (Leng et al., 2003).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD74

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74

Hintergrund: Ia antigen, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, Ii, p33, LN2

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints