Rekombinanter RAGE Antikörper

Produktnummer ABIN7072541

Der Kaninchen Chimeric Anti-RAGE-Antikörper wurde für WB, IHC, ELISA, FACS, IF und SPR validiert. Er ist geeignet, RAGE in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter RAGE Antikörper (ABIN7072541)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser RAGE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Surface Plasmon Resonance (SPR)

Klon: 2A11

Produktdetails anti-RAGE Antikörper

Verwendungszweck: Anti-RAGE [2A11], Rabbit IgG, kappa

Spezifität: 2A11 is specific for the V-domain of human advanced glycosylation end product-specific receptor. It recognises both the glycosylated and non-glycosylated forms.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: Human RAGE protein.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: ELISA confirmed the specificity of 2A11 for the V-domain of RAGE, with a binding affinity (Kd) of 7.6 nM. ELISA was also used to confirm that the binding of 2A11 to RAGE is glycosylation independent. In SPR experiments the Kd of 2A11 binding to RAGE was determined as 0.38 nM. Flow cytometry and immunochemistry were used to visualise the binding of 2A11 to HEK-RAGE cells.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu RAGE

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Andere Bezeichnung: RAGE

Hintergrund: AGER, advanced glycosylation end product-specific receptor, receptor for advanced glycosylation end products

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteine