Rekombinanter CD35 Antikörper

Produktnummer ABIN7072539

Dieses Anti-CD35-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD35 in WB, IF und ELISA. Geeignet für Human.

Kurzübersicht für Rekombinanter CD35 Antikörper (ABIN7072539)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), ELISA

Klon: E11

Produktdetails anti-CD35 Antikörper

Verwendungszweck: Anti-CR1 [E11], Rabbit IgG, kappa

Spezifität: E11 is reactive to neutrophilic granulocytes and eosinophils, monocytes, macrophages, lymphocytes, erythrocytes, dendritic reticulum cells, tonsil tissue section, spleen tissue section, lymph node tissue section, gut tissue section, kidney podocytes and kidney tissue sections, chronic lymphocytic leukemia, acute myelomonocytic leukemia, acute monocyte leukemia, B cell lines (JY, MST, BR17), EBV-infected B cell cultures, myeloid cell lines (U937, HL60, THP 1-0).

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: BALB/c mice were immunised with cells from acute monocytic leukemia patients.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: E11 fluorescence staining of normal blood cells was detectable on 90 % of monocytes, 95 % of neutrophils, and 14 %-24 % of lymphocytes (Hogg et al, 1984). Assay of E11 fluorescence-stained monocytes and neutrophils by EC3b rosette formation indicated that nearly all of the E11-stained cells formed rosettes with EC3b (Hogg et al, 1984). Treatment of erythrocytes, monocytes, neutrophils, and lymphocytes with E11 did not result in any detectable loss of CR1 activity as measured by EC3b rosette formation (Hogg et al, 1984). Immunofluorescence was used to determine E11 reactivity to a variety of cells, tissue sections, leukemias, and cell lines (Hogg et al, 1984). E11 has been used in ELISA experiments to quantify the amount of recombinant CR1 proteins (Park et al, 2013).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Andere Bezeichnung: CR1

Hintergrund: CD35, Type 1 complement receptor, C3b/C4b receptor

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity