Rekombinanter Integrin beta 2 Antikörper

Produktnummer ABIN7072533

Dieses Anti-Integrin beta 2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Integrin beta 2 in FACS, IP, ELISA, IF und RIA. Geeignet für Human und Rhesusaffen.

Kurzübersicht für Rekombinanter Integrin beta 2 Antikörper (ABIN7072533)

Target: Integrin beta 2 (ITGB2)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Rhesusaffen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Integrin beta 2 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), ELISA, Immunofluorescence (IF), Radioimmunoassay (RIA)

Klon: 1B4

Produktdetails anti-Integrin beta 2 Antikörper

Verwendungszweck: Anti-CD18 [1B4], Mouse IgG2a, kappa

Spezifität: Specific to the beta chain of the leukocyte integrin beta-2 family. This is found in LFA-1, Mac-1 and p150.95 among others. Binds an epitope distinct from the C3bi binding site. Has been shown to recognise both the human and Rhesus monkey antigen.

Kreuzreaktivität: Rhesusaffen

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Aufreinigung: Protein A affinity purified

Immunogen: Blood was obtained from 4 helathy donors and the mononuclear cells harvested and grown. A BALB/c mouse was injected with 10^6 monocytes three times at monthly intervals, and four times with 5x10^6 monocytes oon each of the four days before fusion. The spleen cells were then fused to P3U1 myeloma cells to generate hybridomas and RIA was used to identify lines of interest.

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: The main application 1B4 has been proposed for is its anti-inflammatory activity. It has been shown to work as a Fab fragment prepared by papain digestion (Wright et al, 1983). Its binding to antigen was confirmed by RIA (Van Voorhis et al, 1983). 1B4 has been shown to work in Rhesus monkeys, and has reduced immunogenicity once humanized. 1B4 is also compatible with immunofluorescence which has been used to demonstrate that the fine specificity of the combining sites was not altered by humanization (Singer et al, 1993). It has been shown to prevent polymorphonuclear (PMN)-dpepndent pathology in animal models of hemorrhagic shock, dermal inflammation, reperfusion injury, IBD and meningitis (Vedder et al, 1988, Vedder et al, 1990, Mileski et al, 1990, Carlos et al, 1990, Tuomanen et al, 1989, Arfors et al, 1987, Wallace et al, 1991, Wallace et al, 1992). The glycosylation patterns of 1B4 have also been studied in detail using NMR, ES-MS, HPAEC-PAD mapping and glycosyl composition analysis (Yu Ip et al, 1994).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Andere Bezeichnung: CD18

Hintergrund: Integrin beta-2, Cell surface adhesion glycoproteins LFA-1/CR3/p150.95 subunit beta, Complement receptor C3 subunit beta, ITGB2, MFI7, IB4, m1B4, mIB4

UniProt: P05107

Pathways: NF-kappaB Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation