Rekombinanter BTLA Antikörper

Produktnummer ABIN7072471

Dieses Hamster Chimeric-Antikörper erkennt spezifisch BTLA in WB, FACS, IP, Func und BP. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für Rekombinanter BTLA Antikörper (ABIN7072471)

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Hamster

Klonalität: Chimeric

Konjugat: Dieser BTLA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Blocking Peptide (BP)

Klon: HMBT-6B2

Produktdetails anti-BTLA Antikörper

Verwendungszweck: Anti-BTLA [HMBT-6B2], Hamster (Armenian) IgG, kappa

Spezifität: This antibody is specific for B and T lymphocyte attenuator (BTLA), which functions as a negative regulator of T cell activation and proliferation, and attenuates B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).

Produktmerkmale:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising Armenian hamsters with mouse BTLA:Fc fusion protein.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody has been used in immunoblot analyses to confirm that B and T lymphocyte attenuator (BTLA) regulates T cell activation through interaction with herpesvirus entry mediator (HVEM) (Sedy et al, 2005), as well as in FACS and yeast display technology to demonstrate that BTLA exhibits structural and expression polymorphisms and is highly induced in anergic CD4+ T Cells (Hurchla et al, 2005). In a mouse conjunctivitis model, treatment with this antibody during the induction phase has been shown to decrease B-cell population, upregulate Th2 cytokine production and increase the conjunctival eosinophile numbers (Ishida et al, 2012). However, treatment with this antibody during the effector phase does not affect the development of experimental conjunctivitis (Ishida et al, 2012).

Kommentare:

This chimeric hamster (Armenian) antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu BTLA

Target: BTLA (B and T Lymphocyte Associated (BTLA))

Andere Bezeichnung: BTLA

Hintergrund: CD272, B and T lymphocyte attenuator, B- and T-lymphocyte attenuator, B and T lymphocyte associated protein, B- and T-lymphocyte-associated protein, BTLA1, FLJ16065, MGC129743

UniProt: Q7TSA3

Pathways: Cancer Immune Checkpoints