Rekombinanter CTLA4 Antikörper

Produktnummer ABIN7072467

Dieses Anti-CTLA4-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CTLA4 in IF und BR. Geeignet für Maus.

Kurzübersicht für Rekombinanter CTLA4 Antikörper (ABIN7072467)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CTLA4 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Blocking Reagent (BR)

Klon: 9D9

Produktdetails anti-CTLA4 Antikörper

Verwendungszweck: Anti-CTLA-4 [9D9], Rabbit IgG, kappa

Spezifität: The antibody is specific for CTLA-4.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Aufreinigung: Protein A affinity purified

Immunogen: The antibody was raised against CTLA-4.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: The antibody has been used to block CTLA-4 and has been shown to enhance T-cell immunity against tumours (Quezada et al, 2006). 9D9 also has an anti-B16/BL6 melanoma effect in non-irradiated tumour-bearing recipients when given as combinatorial therapy with Gvax (Peggs et al, 2009). Selby et al. (PMID: 24777248) show how conversion of the original mouse IgG2b to a mouse IgG2a greatly increases anti-tumor activity of the 9D9 antibody in both MC38 (C57BL/6 mice) and CT26 (BALB/c mice) models. This mechanism both involves blockade of CTLA4 as well as depletion of tumor-infiltrating regulatory T-cells. Fc Silent antibodies were shown to have reduced anti-tumor activity as they did not deplete tumor infiltrating regulatory T-cells, however they lead to expansion of peripheral Tregs.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CTLA4

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Andere Bezeichnung: CTLA-4

Hintergrund: CD152, CTLA4, Cytotoxic T-Lymphocyte Associated Protein 4, Insulin-Dependent Diabetes Mellitus 12, Celiac Disease 3, Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4, Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Fo

UniProt: P09793

Pathways: Cancer Immune Checkpoints