Rekombinanter IL-10 Antikörper

Produktnummer ABIN7072455

Dieses Anti-IL-10-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von IL-10 in IHC, ELISA, IP, Neut, IF und Crys. Geeignet für Human.

Kurzübersicht für Rekombinanter IL-10 Antikörper (ABIN7072455)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser IL-10 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Neutralization (Neut), Immunofluorescence (IF), Crystallization (Crys)

Klon: 9D7

Produktdetails anti-IL-10 Antikörper

Verwendungszweck: Anti-IL-10 [9D7], Rabbit IgG, kappa

Spezifität: The six 9D7 Fab CDR loops interact with a discontinuous IL-10M1 epitope comprised of residues 71-83 and 125-137. 9D7 non-competitively antagonises IL-10R1 binding.

Produktmerkmale:

Original Species of Ab: Rat

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63-AG8.653, is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: 9D7 has been used in ELISA experiments in order to specifically detect IL-10 and show higher circulating levels of IL-10 in patients with acute EBV-induced mononucleosis (Taga 1995). Further, 9D7 has been used to immunoprecipitate IL-10 from the supernatants of melanoma cells in order to study the production of IL-10 by melanoma cells (Qiyuan Chen 1994). A fab fragment derived from 9D7 has been used to study the noncompetitive antibody neutralisation of IL-10 by x-ray crystallography (Josephson 2002).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Andere Bezeichnung: IL-10

Hintergrund: Human cytokine synthesis inhibitory factor,GVHDS, IL10, Interleukin 10, Interleukin-10, IL10A, TGIF, JES3-9D7, JES3 9D7,

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints